@article{86341,
  keywords = {Animals, Diffusion, Trans-Activators, Models, Biological, Recombinant Fusion Proteins, Homeostasis, Morphogenesis, Body Patterning, Cell Nucleus, Mitosis, Oocytes, Drosophila melanogaster, Homeodomain Proteins, Fluorescence Recovery After Photobleaching},
  author = {Thomas Gregor and Eric Wieschaus and Alistair McGregor and William Bialek and David Tank},
  title = {Stability and nuclear dynamics of the bicoid morphogen gradient.},
  abstract = { Patterning in multicellular organisms results from spatial gradients in morphogen concentration, but the dynamics of these gradients remain largely unexplored. We characterize, through in vivo optical imaging, the development and stability of the Bicoid morphogen gradient in Drosophila embryos that express a Bicoid-eGFP fusion protein. The gradient is established rapidly (approximately 1 hr after fertilization), with nuclear Bicoid concentration rising and falling during mitosis. Interphase levels result from a rapid equilibrium between Bicoid uptake and removal. Initial interphase concentration in nuclei in successive cycles is constant (+/-10\%), demonstrating a form of gradient stability, but it subsequently decays by approximately 30\%. Both direct photobleaching measurements and indirect estimates of Bicoid-eGFP diffusion constants (D },
  year = {2007},
  journal = {Cell},
  volume = {130},
  pages = {141-52},
  month = {07/2007},
  issn = {0092-8674},
  doi = {10.1016/j.cell.2007.05.026},
  language = {eng},
}